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The 2 protein bands from 40 kDa to thirty kDa ended up absent just after 24 h incubation at forty five °C. For the rest of the bands, numerous concentrations of all three proteases retained protein bands corresponding to their unique molar masses (Figure 6a,b). The presence of bands at low molecular bodyweight in fish roe just before hydrolysis was surprising. Inside the report by Ghelichi et al. [eight], minimal peaks of a relatively reduced molecular fat selection (3–14 kDa) were being noticed in defatted carp roe homogenate, most likely on account of an autolytic method transpiring in fish roe and/or even the defatting method resulting in the formation of reduced-molecular-bodyweight peptides.
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